Application

Activity Assay Analysis: A representative lot detected that S100A8/MRP-8 reduces mouse neutrophil accumulation in dorsum-raised air pouchs in the presence of MSU crystals (Ryckman, C., et al. (2003). ARTHRITIS & RHEUMATISM. 48(8):2310–2320).
Immunohistochemistry Analysis: A representative lot detected S100A8/MRP-8 in mouse arthritic paw tissue (Cesaro, A., et al. (2012). PLOS ONE. 7(9):e4578).

This Anti-S100A8/MRP-8 Antibody (Azide Free) is validated for use in Western Blotting and Immunohistochemistry and Activity Assay for the detection of S100A8/MRP-8 (Azide Free).

Research Sub CategoryImmunoglobulins & Immunology

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

S100A8, also known as Protein S100-A8, Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen (CFAG), Leukocyte L1 complex light chain, Migration inhibitory factor related protein 8 (MRP-8), p8, S100 calcium binding protein A8, or Urinary stone protein band A, and encoded by the gene S100A8/CAGA/CFAG/MRP8, is an important protein that binds both zinc and calcium and plays an important role in the regulation of inflammatory processes and immune response. Found in complex with its partner S100A9, S100A8 facilitates chemotaxis, fatty acid trafficking, cytoskeleton reorganization, and NAPDPH oxidase activation and other intracellular activities, as well as a host of extracellular induced activities such as extracellular proinflammatory, antimicrobial, oxidant scavenging, and apoptotic activities . Additionally S100A8 can act as a potent autoimmunity amplifier and appears to augment various cancers and their spread when over expressed. S100A8 is widely expressed and used as biomarker in patients with inflammatory diseases and as a cancer marker in multiple forms of cancer.

Immunogen

Recombinant protein corresponding to mouse S100A8/MRP-8.

Physical form

Rabbit polyclonal serum without preservatives.

Unpurified

Quality

Evaluated by Western Blotting in human spleen tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected S100A8/MRP-8 in 10 µg of human spleen tissue lysate.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~10 kDa observed. Uncharacterized band(s) may appear in some lysates.